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Accueil Tous les numéros Volume 32 (2025) OCL, 32 (2025) 38 Full HTML
Numéro
OCL
Volume 32, 2025
Minor oils from atypical plant sources / Huiles mineures de sources végétales atypiques
Numéro d'article 38
Nombre de pages 11
DOI https://doi.org/10.1051/ocl/2025035
Publié en ligne 10 décembre 2025

© L. Rojas-Álvarez et al., Published by EDP Sciences, 2025

Licence Creative CommonsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Highlights

  • Proximate composition analysis of mesocarp + exocarp (M+E) showed increased fat and protein contents in mature fruits

  • SFE demonstrated higher oil yield from M+E of mature fruits

  • GC-FID analysis of fatty acids identified oleic acid as the principal constituent

  • SFE at 50 °C/34 MPa efficiently recovered valuable oleic acid and volatiles compounds

1 Introduction

Generally, palms are rich in oils, terpenoids, and phenolic compounds. The fruits of several species contain high levels of healthy oils and bioactive compounds, with terpenoids, such as carotenoids, phytosterols, pentacyclic triterpenes, and tocopherols, standing out (Silviera Agostini-Costa, 2018). The palm Oenocarpus bataua Mart. grows naturally in tropical humid forests and is distributed throughout the Amazon basin, forests of the biogeographic Chocó region in Colombia, the Neotropical humid montane forests of Bolivia, Brazil, Colombia, Ecuador, Guyana, Panama, Peru, and Venezuela, as well as on the island of Trinidad in the Caribbean. According to García et al. (2010), O. bataua is a promising species in the Colombian national strategy for plant conservation. Given its high-quality oil production potential with chemical properties very similar to olive oil, various authors argue that it could be used in a wide range of industrial products (Mushtaq et al., 2019; Vasconcelos dos Santos et al., 2019). O. bataua grows predominatly in lowlands and mountain slopes up to 1400 m a.s.l. It is one of the most common species in tropical rainforests, reaching a height between 10 and 20 m. It typically is the dominant canopy and sub-canopy species (Mushtaq et al., 2019; Copete et al., 2024).

In cosmetics, oils are used as an ingredient in moisturizing formulations, serums, lipsticks, and skin and hair care products due to their nourishing and skin barrier repair properties (Vaughn et al., 2018; Guzmán and Lucia, 2021). Native palms from the Colombian Amazon, such as Euterpe stipitata, Euterpe precatoria, Mauritia flexuosa, and Oenocarpus bataua, are used as ingredients in the production of cosmetic, specifically for use as skin conditioners, emollients, and moisturizers (Bravo et al., 2020; Jiménez-Tamayo et al., 2024). Various authors have found bioactive compounds in O. bataua, including piceatanol, a natural stilbene considered more active than resveratrol (Maruki-Uchida et al., 2018; Jaramillo-Vivanco et al., 2022). Studies carried out by Hernández et al. (2009) and Montúfar-Galárraga and Brokamp (2017) found a sterol fraction represented mainly by Δ 5-avenasterol, a potential antioxidant that could be used as a marker of authenticity; β-sitosterol, stigmasterol and a high concentration of α-tocopherols (vitamin E). These are compounds that are used in cosmetic products as antioxidants, skin and hair conditioning agents, and skin protectants (Oliveira and Rodrigues, 2018; Carvalhães-Lago et al., 2021).

Ocampo-Duran et al. (2013), determined the lipid profile of O. bataua oil, reporting that oleic acid represented the largest proportion (81%), followed by palmitic acid (12%), stearic acid (4%) and essential fatty acids, including linoleic (2.5%) and linolenic (1%) acids. Cardona-Jaramillo et al. (2019) reported that triglycerides stand out as the main lipid components identified in O. bataua oil. These are found in the outermost layer of the epidermis, and their action is to prevent water loss. They act as an emollient, enabling them to be used as a natural ingredient in cosmetic products.

There are various methods for oil extraction, among which the most innovative is the supercritical fluid method (SCF), which represents a sustainable alternative to traditional extraction systems (Kate et al., 2016; Zhou et al., 2021). Supercritical carbon dioxide (CO2) is a non-polar solvent frequently used as it is: non-toxic, non-flammable, non-corrosive, inert, colorless, inexpensive, easily removed, leaves no residue, is reusable, readily available, and has the potential to extract heat-sensitive compounds, thus reducing the loss of the functional properties of the bioactive compounds present in oils (Zhang et al., 2018; Narváez-Cuenca et al., 2020; Arumugham et al., 2021).

Currently, there are no comparative studies on the yield and chemical composition of O. bataua oil at different stages of fruit maturity using the SCF oil extraction method in this species; however, studies on the yield and chemical composition of oil are reported for other palm species, including Orbignya phalerata (de Oliveira et al., 2019), Oenocarpus mapora (Muñoz et al., 2022), and Oenocarpus bacaba (Pinto et al., 2018). The SCF extraction efficiency depends on solubility variation with temperature (40 to 80 °C) and pressure (20 to 35 MPa) (Cunha et al., 2019; Turola-Barbi et al., 2020). This method has been used to evaluate the extraction yield and quality of the lipid profile (fatty acids, sterols, tocopherols, and volatile compounds), plant pigments (non-polar compounds), and bioactive compounds (moderately polar compounds) (Radzali et al., 2020). There are several studies in the literature on the bioactive compounds present in O. bataua oil (Jaramillo-Vivanco et al., 2022; Vasconcelos dos Santos et al., 2019); however, for the Bajo Calima area (Colombia) that belongs to the Chocó biogeographic region, few studies have reported the chemical composition and volatile compounds of this oil, and thus, it is underutilized despite its potential benefits.

The aim of this research is to carry out the morphological characterization of the ripe and semi-ripe fruits of O. bataua; a proximate analysis of the pulp; determine the yield of the oil extracted by the SCF method using CO2 from ripe and semi-ripe fruits; and to determine the quality of O. bataua according to its chemical composition. This chemical composition includes the fatty acid profile by gas chromatography with flame ionization detection (GC/FID). The volatile profile analysis used gas chromatography-mass spectrometry (GC/MS). This study aims to encourage enhancements in traditional extraction methods and promote sustainable use to improve the economy and social conditions of communities in areas where this species grows naturally.

2 Materials and methods

2.1 Study area and sampling

The study was carried out in Bajo Calima, Buenaventura, located in the Department of Valle del Cauca, Colombia, in the area of influence of the Tropical Forest Center (CFT, for its Spanish acronym). The vegetation corresponds to a secondary forest where palms prevail under natural growth conditions, i.e., not planted or managed. According to the ecological classification of Holdridge (1978), this area corresponds to the life zone “very humid tropical forest in transition to tropical pluvial forest” (bmh-T/bp-T), with an average annual temperature of 26 °C, relative humidity of 88%, a short number of hours of solar brightness per year (712 h / year), and average annual precipitation of 7500 mm / year.

Nine palms were selected for this study; and all were in their reproductive state (young and adult) in order to ensure variability of fruits in terms of age. Fruits collected were selected and counted (number of healthy fruits), packed in plastic cloth-type sacks, and transported to the collection center at CFT.

2.2 Fruit samples for morphological description

All the fruits of each raceme were weighed (g), and samples were randomly selected from each one according to their ripeness stage as follows: 30 semi-ripe (dark violet with small shades of green) and 30 ripe (deep violet) fruits from a total of nine palms. The morphological evaluation of each selected fruit was carried out, including measurement of the length of fruit from the base to the apex (cm) and the diameter in the central part (cm) using a digital caliper; these were then weighed (g).

2.3 Fruit treatment for oil extraction

Of the nine palms evaluated in this research, only the pulp and peel of ripe (two palms) and semi-ripe (two palms) fruits were selected. Ripe and semi-ripe fruits were subjected to a heat-softening process by immersing them in water at 50 °C for 1 h; then, the mesocarp and exocarp (M+E) were separated from the seed. All the samples were weighed on a FENIX-15 KG scale (China) and stored in trays to be dried in an oven at 50 °C for approximately 24 h. Subsequently, the samples were stored in polyethylene bags at −10 °C. Mesocarp and exocarp samples of both maturity stages were uniformly mixed for oil extraction.

2.4 Proximal chemical analysis of mesocarp + exocarp

The proximal chemical analysis was carried out using mesocarp and exocarp samples per ripening stage, measuring, variables including moisture, ash, lipids, and proteins as percentages according to the official methods of AOAC (2005). The percentage of lipids was determined using the Randall method, also known as Soxtec or immersion method, is a lipid extraction technique, a modification of the standard Soxhlet extraction method, using the Velp ScentificaTM SER 148 Solvent Extractor equipment (Italy).

2.5 Supercritical fluid extraction

Oil extraction was carried out with the Waters SFE 500 equipment (USA), according to the process diagram (Fig. 1), using carbon dioxide (CO2) as a solvent of 99.9% purity (Cryogas, Colombia) available in 25 kg cylinders. Approximately 150 g of dry matter from each sample, previously ground in an electric mill, was used for extraction. To determine the size of the particles, a set of sieves, from sieve No. 4 (4.76 mm) to sieve No. 200 (0.075 mm), ware used, considering the Colombian regulations NTC 77 “Test method for the analysis by sieving of fine and coarse aggregates.”

A kinetic curve was elaborated with intermediate conditions, selected from a previous test (not included in this study) where the oil performance was evaluated at pressure intervals of 30 to 38 MPa and temperature ranges from 40 to 60 °C. Based on these results, a mean pressure and temperature of 34 MPa and 50 °C, respectively, were identified to carry out the pilot experiment, maintaining a constant flow of 30 g CO2/min with a density of 893.91 kg/m3 for 320 min of extraction. The volume of the extraction vessel was 500 ml with an internal height of 21.5 cm and internal diameter of 5.73 cm; a ratio of height to diameter of 1:3.75. The extracted oil was stored in amber bottles and refrigerated at 4 °C to analyze fatty acids and volatile compounds. Oil yield was calculated as the weight of oil extracted over the total weight of M+E and expressed as the percentage of oil extracted (Eq. (1)), according to the methodology described by Pantoja-Chamorro et al. (2017a).

%Oilyieldextracted=(Weightofoilextracted,  ingTotalweightofmesocarp+exocarp,  ing)×100.(1)

For the kinetic modeling of supercritical extraction curves, the model of Sovová et al. (2006) was used, for each maturity stage. The semi-empirical model of Sovová et al. (2006) considers the resistance in mass transfer within the solvent as insignificant. The model simulates the extraction process of two solutes deposited in a continuous-flow through the following equation (Eq. (2))

e=e1[1-exp(-k1q)]+k2q,(2)

where “e” is the extraction yield (w/w), “q” the relationship between dissolvent/raw material (w/w), and e 1, k 1, k 2 are the adjustable parameters of the model.

thumbnail Fig. 1

Flow process diagram of the supercritical fluid extraction method using CO2. Source: Pantoja-Chamorro et al. (2017b).

2.6 Fatty acids composition of O. bataua oil by GC/FID

The chemical composition of the fatty acids profile for M+E (FAME) oil was determined by gas chromatography with a flame ionization detector (GC/FID). Fatty acids were analyzed as methyl esters of fatty acids present in the sample; the method of comparing their retention times with those of the standard 37 Component FAME Mix certified reference material (AccuStandard, Inc., 125 Market Street, New Haven, CT 06513) analyzed under the same chromatographic conditions was used. The chromatographic analysis was carried out using a gas chromatograph (GC) AT 6890N (Agilent Technologies, PA, California, USA) with a flame ionization detector (FID). The injection was performed in split mode (50:1), and Viny: 2 μ L. The compounds were separated on a DB-23 Column (J & W Scientific, Folsom, CA, USA) of 50%-cyanopropyl-methylpolysiloxane with the following dimensions: 60 mx 0.25 mm × 0.25 μ m.

2.7 Analysis of volatile compounds by HS-SPME/GC-MS

The volatile profile was obtained by gas chromatography coupled to mass spectrometry (GC-MS) operated in full scan mode. The analysis of the aroma-responsible compounds, i.e., volatile organic compounds (VOCs), was carried out by simultaneous extraction-concentration of the volatile compounds in the sample using a 65 μ m thick PDMS/DVB-coated fused silica fiber with the following conditions: equilibrium time 10 min; extraction time 30 min; extraction temperature 60 °C; desorption time in the chromatographic port 10 min; desorption temperature at the injection port 250 °C (Agilent Technologies AT 6890 gas chromatograph coupled to a mass selective detector MSD, AT 5973N), operated in full scan mode; injection was performed in splitless mode with a commercial HS-SPME device. A chromatographic column DB-5MS (J & W Scientific, Folsom, CA, USA) of 5%-Ph-PDMS of dimension 60 mx 0.25 mm × 0.25 μ m was used. The presumptive identification of the compounds present in the samples (>0.1%) was based on their mass spectra (EI, 70 eV), fragmentation patterns, and comparison of the mass spectra with those included in the Adams, Wiley, and NIST databases and available reference substances.

2.8 Statistical analysis

The data were analyzed through a variance analysis using the Minitab® 19.1 program (USA), with a statistical significance (α) of P<0.05. A sampling design was used for descriptive statistics, employing the quantitative variables for fruits at each ripeness stage. Furthermore, the mean and standard deviation were determined for the percentage of mesocarp, exocarp, and seed, and the proximal M+E analysis of the O. bataua fruits.

3 Results and discussion

3.1 Morphological characterization of O. bataua fruits

Semi-ripe fruits have a dark violet color with small shades of green (Hernández-Gómez, et al., 2018) and ripe fruits have an intense violet color (Cevallos et al., 2013). According to Hernández-Gómez et al. (2018), the ripe stage of O. bataua fruits is recommended for oil extraction since these fruits have reached adequate organoleptic and physicochemical characteristics, and the separation of the exocarp, mesocarp, and seed for processing purposes is easier at this stage. In the African oil palm Elaeis guineensis, the detachment and change in color of the fruits are considered ripening indicators, criteria for planning its harvest (Henson, 2012).

Table 1 presents the morphological characteristics of the O. bataua fruits. The average length, diameter, and weight are detailed separately for semi-ripe and ripe fruits, with measurements of 3.63 cm, 2.29 cm, and 13.24 g for semi-ripe fruits and 3.64 cm, 2.40 cm, and 14.01 g for ripe fruits, respectively. The means of the three variables are slightly similar in the two ripening stages, indicating that the fruit in both has likely completed cell differentiation, reaching its final size. In the Peruvian Amazon, Quispe-Jacobo et al. (2009) reported average length (3.35 cm) and diameter (2.25 cm) values similar to the ones found in the current study; however, weight values were lower (11.06 g). Likewise, Hernandez-Gómez et al. (2018) reported similar values in the Colombian Amazon for length (3.36 cm) and diameter (2.57 cm), along with lower values for weight (10.08 g).

In a sample of 80 fruits (Tab. 2), the proportions of seed, mesocarp, and exocarp were determined from the weight (g) of the fruit and expressed as a percentage. Results show that the seed represents the highest percentage (68.57%) of the fruit, followed by the exocarp (20.14%) and, the mesocarp (11.36%). The combined percentage for exocarp and mesocarp (M+E) was 31.5%. These results are similar to those reported by Cotos et al. (2020), who found average values of 63.08% for the seed and 35.09% for the M+E. However, the M+E values of O. bataua were higher than those reported for Euterpe precatoria (açaí), which constitutes only 17% of the fruit (Ortega-Romero et al., 2015), demonstrating a greater potential than açaí, a species that is already widely recognized at the agro-industrial level.

Table 1

Morphological evaluation of Oenocarpus bataua fruits in two maturity stages.

Table 2

Morphological evaluation of ripe Oenocarpus bataua fruits.

3.2 Proximate chemical analysis of the mesocarp and exocarp of O. bataua fruits

Table 3 presents the results of the analysis of fats (21.66 ± 2.70%), ash (1.95 ± 0.05%), and proteins (6.7 ± 0.39%), which were higher in ripe fruits; however, moisture was higher in semi-ripe fruits (5.52 ± 0.05%). The fat content in the two stages was different, registering a higher concentration in the ripe stage (21.66 ± 2.70%). The fat content values in the current study are similar to those reported by Quispe-Jacobo et al. (2009), who registered 21.77 ± 0.176%, and higher than those found by Darnet et al. (2011), who reported 14.4 ± 0.04%.

Ash values indicate the presence of minerals. In this study, there was no significant difference for p=0.0067, registering lower values than those reported by De Marcano et al. (2004) (2.21 ± 0.09%). Protein content in the two ripening stages shows a highly significant difference, with the ripe stage showing the highest proportion (6.7 ± 0.39%); however, it is a lower value than that reported by De Marcano et al. (2004) (8.17 ± 0.04%) but higher than the one found by Darnet et al. (2011) (3.32 ± 0.26%). The F-test of the ANOVA for both ripening stages was highly significant (p<0.0001).

Table 3

Proximal composition of the mesocarp (pulp) + exocarp (peel) of semi-ripe and ripe Oenocarpus bataua fruits.

3.3 Supercritical CO2 extraction yield

For this study, the average particle size of the M+E of ripe and semi-ripe fruits obtained was 2.23 ± 1.84 mm. Figure 2 shows the oil extraction kinetics curves of the two fruit ripening stages (semi-ripe and ripe) as a function of time (min) on the response variable yield (%) under the following conditions: T=50 °C and P=34 MPa. Sovová et al.’s (2006) was applied to described the kinetics of supercritical CO2 extraction. The adjusted model parameters are shown in Table 4.

The oil yield extracted from semi-ripe fruits (12.48%) was lower than that of ripe fruits (17.99%) after 320 min of extraction. When comparing the two curves, the percentage of oil extracted from ripe fruits increases progressively from the beginning and persisted until 220 min, recovering to 86.61% (extraction yield of 15,58). However, the data fit falls short in predicting the inflection point. By contrast, after 140 min, the percentage of oil decreased significantly for semi-ripe fruit, achieving 80% of the oil contained in the M+E of the O. bataua fruit during this extraction time. The model by Sovová et al. (2006) described 97.24% (ripe fruit) and 96% (semi-ripe fruit) oil extraction kinetics of M+E of the O. bataua fruit at 34 MPa/50 °C, indicating that it is a suitable model. The model simulates the extraction of two solutes. The first solute in the yield e 1 is completely mixed with the solvent, and the solubility of the second solute in the solvent is k 2, have lower values, which could indicate that a certain amount of M+E oil from O. bataua fruit was partility dissolved in SC- CO2 and became a component of the second solute. This is shown in Figure 2: after 140 min, the yield begins its decrease, showing that the solute has a higher affinity with the solid matrix than with the solvent (Sovová et al., 2006).

According to Pantoja-Chamorro et al. (2017a), at the industrial level, it is not profitable to carry out processes that involve long extraction times; therefore, according to this study, a significant percentage of oil (76.82%) was recovered from ripe fruits after 180 min, indicating an optimal extraction time. In a study carried out with Oenoarpus bacaba, Pinto et al. (2018) found that the highest yield of 60.39% was obtained at 60 °C/40 Mpa under slightly similar conditions. In contrast, Chañi-Paucar et al. (2021) with Mauritia flexuosa, only achieved a yield of 36.2% employing the same conditions (60 °C/40 MPa). Further, for Euterpe oleracea, the best yield of 45.4% was obtained at 70 °C/49 MPa (De Cássia Rodrigues et al., 2015).

thumbnail Fig. 2

Supercritical extraction kinetic curve of the mesocarp+exocarp ripe and semi-ripe O. bataua fruits oil: 34 MPa/50 °C.
Table 4

Parameters of equation (2) for the extraction yield from mesocarp+exocarp of ripe and semi-ripe O. bataua fruits with CO2: 34 MPa/50 °C.

3.4 Fatty acid composition of O. bataua oil

Table 5 shows the fatty acid composition of M+E oil extracted from semi-ripe and ripe fruits, predominating oleic acid (87.6% and 81.2%, respectively) followed by palmitic acid (8.2% and 11.9%, respectively) and unsaturated fatty acids, especially linoleic ω−6 (2.1% and 2.4%, respectively) and linolenic ω−3(0.6% only found in ripe fruits). These are considered essential fatty acids. Therefore, this oil has the potential to be used in the food, cosmetics, and pharmaceutical industries. Unsaturated fatty acids, mainly oleic and linoleic acids, which include monounsaturated (MUFA) and polyunsaturated (PUFA) acids, represent 90.3% of the total fatty acids in the M+E oil extracted from semi-ripe fruits and 84.9% of the total fatty acids in the M+E oil extracted from ripe fruits. The results indicate that the oil obtained is of high quality regardless of the ripeness stage of the O. bataua fruits, especially in unsaturated fatty acids.

The saturated fatty acids (SFA) in the semi-ripe and ripe fruits analyzed represent 9.5% and 14.4%, respectively, with palmitic and stearic acids being the most prevalent. In the cosmetics industry, palmitic, oleic, and stearic acids are used in dermo-cosmetics, given their effectiveness as emollients (Ogorzalek et al., 2024). By using other extraction methods to obtain the oil of O. bataua, Vasconcelos dos Santos (2019) reported a slightly lower percentage of oleic acid (71.79%) but higher values of stearic (13.53%) and linoleic (4.72%) acids. Likewise, Pereira et al. (2020) reported slightly lower values of oleic acid (74.18%) and similar percentages of linolenic acid (0.51%). For their part, Carrillo et al. (2018) and Serra et al. (2019) found slightly similar percentages of oleic acid (82.03% and 78.46%, respectively). The fatty acid composition of O. bataua oil is similar to olive oil, mainly in the oleic acid content, an important source of unsaturated fatty acids (Carrillo et al., 2018).

In this study, O. bataua oil extracted using the supercritical CO2 extraction method was compared with that extracted from other palm species using this same method because there are no reports in the literature for O. bataua. Therefore, it is evident that there are differences in the composition of fatty acids and other bioactive compounds. Pinto et al. (2018) reported 61.59% of MUFA for Oenocarpus bacaba oil extracted at 29 MPa/40 °C, with oleic acid predominating (61.16%), and 11.58% of PUFA. For Oenocarpus distichus, Cunha et al. (2019) evaluated extraction conditions with pressures between 15−35 MPa at 50 °C and 19−42 MPa at 60 °C for 180 min. The authors found no significant difference in the qualitative composition of MUFA and PUFA fatty acids. In terms of quantity, oleic fatty acid stood out regardless of the extraction conditions (≅ 66%). Likewise, de Oliveira et al. (2019) found that in the oil extracted with supercritical CO2 from Orbignya phalerata seeds, the fatty acid composition varied very little depending on the temperatures (40−80 °C) and pressures (25 and 35 MPa) evaluated. Therefore, there was no correlation between pressure and temperature with the concentration of fatty acids. For their part, De Cássia-Rodrigues et al. (2015) determined the fatty acids in the fruit of Euterpe Oleracea under pressure conditions between 15−49 MPa and temperature between 50 and 70 °C, and found that the standard deviation was lower than 1.8%. Furthermore, Teixeira-Costa et al. (2016) evaluated the fatty acid profile in two varieties of tucuma, Astrocaryum aculeatum and Astrocaryum vulgare, with temperatures between 40−60 °C. They found that unsaturated acids are predominant, highlighting oleic acid, with average ranges between 73.81 and 71.97% for Astrocaryum aculeatum, regardless of the extraction temperature. Similarly, for Astrocaryum vulgare, the presence of palmitic acid did not vary, indicating a low influence of temperature on the quality of the oil obtained from both species. Pinto et al. (2018), in a study with Oenocarpus bacaba at temperatures of 40 and 60 °C and pressure between 12 and 42 MPa, found that an increase in temperature and pressure improved oil yield. However, the lipid profile composition did not show significant differences in the predominant fatty acids with respect to changes in pressure and temperature. The increase in temperature affects the density of the supercritical fluid, which reduces extraction efficiencies. Nonetheless, it increases the volatility of the compounds, which leads to improved mass transfer and solubility of the components (Cvitković et al., 2024).

Table 5

Fatty acids composition of oil extracted from the mesocarp+exocarp of ripe and semi-ripe O. bataua fruits.

3.5 Chemical composition analysis of the volatile fraction of oil extracted from M+E of O. bataua ripe fruits

The aroma and flavor of virgin oils depend mainly on their volatile compounds. However, there is insufficient literature on studies identifying volatile compounds for O. bataua oil. Hence, in the current study, the HS-SPME/GC-MS analysis of the volatile fraction of O. bataua oil obtained by SC-CO2 indicated the presence of 11 compounds listed in Table 6. The volatile fraction registered mainly alcohols (3), acids (3), terpenes (2), and aldehydes (3). Ethanol is the most abundant (33.2%), followed by phenylethyl alcohol (17.8%) and 3-methylbutanoic acid (16.1%). Limonene (8.4%) and 2-methylbutanoic acid (7.0%) were also found in a smaller proportion. In olive oil, volatile compounds have been widely studied and are responsible for various sensory properties, which can influence quality. Sensory defects are commonly related to chemical oxidation and exogenous enzymes involved in microbial activity (Gomes da Silva et al., 2012). The flavor and aroma of olive oil are characterized by being mild, fruity, and slightly bitter (Fernandes-Silva et al., 2013); Kiritsakis (1998) reported that volatile organic compounds belonging to chemical classes such as aliphatic and triterpenic alcohols, aldehydes, ketones, ethers, and esters are responsible for these flavors and aromas. Phenylethyl alcohol (PEA) is an aromatic compound found naturally in flowers, including roses, geraniums, orange blossoms, hyacinths, champak, and grape skin. It is valuable at the industrial level since it provides various flavors and odors (Perestrelo et al., 2006) and has antioxidant and microbial properties. In a study by Sirilun et al. (2017), the authors found that PEA reduces microbial growth in cosmetic formulations during storage, highlighting it as an alternative to traditional chemical preservatives. Oenocarpus bataua oil is characterized by having a soft, sweet, and slightly fruity aroma; possibly, its aroma is influenced by this aromatic alcohol. 3-Methylbutanoic acid, also known as isovaleric acid, and its volatile esters, have pleasant aromas and are used in perfumery. According to Perez-Santana et al. (2020), in a study carried out with cacao, 3-methylbutanoic acid, 2-methylbutanoic acid, and phenylacetaldehyde are the components responsible for the aroma. On the other hand, in olive oil, 3-methylbutanoic and 2-methylbutanoic acids are generally associated with an unpleasant “rancid” odor and are usually related to damage or improper storage of olives or due to microbial fermentation (Cecchi et al., 2022). In this study, the presence of these isovaleric acids probably indicate a slight deterioration of the oil. Limonene is a monocyclic monoterpene compound primarily found in citrus fruit peels and is a natural antioxidant used as a flavor and fragrance additive in different consumer products such as cosmetics and skin care, soap, detergents and beverages (Himed et al., 2019). The presence of limonene in O. bataua oil could be contributing to the citrus note of its fruity aroma.

Table 6

Identification of volatile compounds from Oenocarpus bataua oil obtained from the mesocarp+exocarp of ripe fruits.

4 Conclusions

The characterization of the O. bataua palm fruit confirmed its potential for agro-industrial use, using physiologically ripe fruits since a better extraction of the oil contained in the mesocarp and exocarp can be obtained. The semi-empirical model of Sovová et al. (2006) described 97,24% of the experimental data behavior of extraction kinetics. In the fatty acid profile of the oil extracted from M+E of semi-ripe and ripe fruits, unsaturated fatty acids, such as oleic, palmitic, and linoleic acid ω−6 and linolenic acid ω−3 (only found in ripe fruits), predominate. These are considered essential fatty acids, showing the efficiency of the SC-CO2 method at 50 °C/34 MPa to produce high-quality oil. The high levels of monounsaturated and polyunsaturated fatty acids found in the oil of O. bataua represent a potential usage of the oil present in the mesocarp and exocarp of these fruits as an important natural ingredient in the cosmetics, pharmaceutical, and food industries. The volatile fraction of the oil obtained using the supercritical CO2 method contains chemical compounds, most notably phenylethyl alcohol, 3-methylbutanoic acid, 2-methylbutanoic acid, and phenylacetaldehyde, which, together, most likely provide the sweet, and fruity aroma typical of this oil. Studies aimed at identifying volatile compounds in palm oils obtained by SC-CO2 appear to be limited or non-existent. Therefore, the analysis of volatiles in this study provides the basis for future research on O. bataua oil and other palms of importance in the pharmaceutical, food, and cosmetics industries.

Acknowledgments

This work was supported by Minciencias Colombia, Universidad del Tolima, and Fondo Fundación WWB Colombia para la Investigación.

Conflicts of interest

The authors declare that they have no conflicts of interest concerning this work.

References

Cite this article as: Rojas-Álvarez L, Hurtado-Benavides AM, Mejía-España DF, Sandoval-Aldana AP, Forero-Peña LA. 2025. Lipid profile of Oenocarpus bataua Mart. oil extracted by supercritical fluid-CO2 from fruits in two ripeness stages in Bajo Calima, Buenaventura, Colombia. OCL 32: 38. https://doi.org/10.1051/ocl/2025035

All Tables

Table 1

Morphological evaluation of Oenocarpus bataua fruits in two maturity stages.

In the text
Table 2

Morphological evaluation of ripe Oenocarpus bataua fruits.

In the text
Table 3

Proximal composition of the mesocarp (pulp) + exocarp (peel) of semi-ripe and ripe Oenocarpus bataua fruits.

In the text
Table 4

Parameters of equation (2) for the extraction yield from mesocarp+exocarp of ripe and semi-ripe O. bataua fruits with CO2: 34 MPa/50 °C.

In the text
Table 5

Fatty acids composition of oil extracted from the mesocarp+exocarp of ripe and semi-ripe O. bataua fruits.

In the text
Table 6

Identification of volatile compounds from Oenocarpus bataua oil obtained from the mesocarp+exocarp of ripe fruits.

In the text

All Figures

thumbnail Fig. 1

Flow process diagram of the supercritical fluid extraction method using CO2. Source: Pantoja-Chamorro et al. (2017b).
In the text
thumbnail Fig. 2

Supercritical extraction kinetic curve of the mesocarp+exocarp ripe and semi-ripe O. bataua fruits oil: 34 MPa/50 °C.
In the text

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