OCL 2021, 28, 17DOI: 10.1051/ocl/2020064© O.M. Oluba et al., Hosted by EDP Sciences, 2021

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Research Article

Effects of drying methods on compositional characterization and functional characteristics of Blighia sapida aril oil☆

Effets des méthodes de séchage sur la caractérisation de la composition et les caractéristiques fonctionnelles de l’huile de Blighia sapida aril

Olarewaju M. Oluba¹*, Divine-Favour O. Mbamara¹, Oghenerobor B. Akpor², Feyikemi D. Adebiyi³, Olayinka O. Alabi⁴, Ayoola Shoyombo⁴ and Omorefosa O. Osemwegie²

¹ Department of Biochemistry, Food Safety Toxicology Research Unit, College of Pure Applied Sciences, Landmark University, Omu-Aran, Kwara State, Nigeria
² Department of Microbiology, College of Pure & Applied Sciences, Landmark University, Omu-Aran, Kwara State, Nigeria
³ Department of Chemical Sciences, Joseph Ayo Babalola University, Ikeji-Arakeji, Osun State, Nigeria
⁴ Department of Animal Science, College of Agricultural Sciences, Landmark University, Omu-Aran, Kwara State, Nigeria

* Correspondence: oluba.olarewaju@lmu.edu.ng; olubamike2000@yahoo.co.uk

Received: 21 July 2020 Accepted: 2 November 2020 Published online: 3 March 2021

Abstract

The composition of Blighia sapida fruits is reported to differ based on its origin, variety, and preservation technique. In this study, the effect of drying method on the composition as well as the antioxidant activity of oven-dried and sun-dried B. sapida aril flour and oil were examined using standard procedures. Sun-dried B. sapida aril flour had significantly higher protein and fat content compared to the oven-dried flour. In addition, sun-dried B. sapida aril flour contained a more diverse phytochemical profile compared to the oven-dried flour. However, oven-dried B. sapida aril oil (ODAO) showed a higher percentage of unsaturated fatty acids compared to oil from the sun-dried B. sapida flour (SDAO). On the other hand, SDAO showed significantly higher DPPH scavenging and nitric oxide inhibitory activities compared to ODAO. Based on these findings, sun-drying is recommended for both nutritional purposes and other health-promoting usage such as antioxidant, over oven-drying.

Résumé

La composition des fruits de Blighia sapida diffère selon leur origine, leur variété et leur technique de conservation. Dans cette étude, l’effet de la méthode de séchage sur la composition ainsi que l’activité antioxydante de la farine et de l’huile de B. sapida aril séchées au four et au soleil sont examinés selon des procédures standard. La farine de B. sapida aril séchée au soleil avait une teneur en protéines et en graisses nettement plus élevée que la farine séchée au four. Toutefois, la farine de B. sapida aril séchée au four présente un profil phytochimique plus diversifié que celle séchée au soleil. L’huile de B. sapida aril séchée au four (ODAO) présentait un pourcentage plus élevé d’acides gras insaturés que celle séchée au soleil (SDAO). En outre, l’ODAO a montré des activités de piégeage du DPPH et d’inhibition de l’oxyde nitrique significativement plus élevées que la SDAO. Sur la base de ces résultats, le séchage au soleil pourrait être plus adapté à des fins nutritionnelles, alors que pour d’autres usages favorables à la santé, tels que les antioxydants, le séchage au four semblerait plus approprié.

Key words: Blighia sapida aril flour / Blighia sapida aril oil / food composition / fatty acid profile / antioxidants

Mots clés : Farine de Blighia sapida aril / huile de Blighia sapida aril / composition des aliments / profil des acides gras / antioxydants

☆

Contribution to the Topical Issue “Minor oils from atypical plant sources / Huiles mineures de sources végétales atypiques”.

1 Introduction

The challenge of food security, coupled with increasing global population, has necessitated the search for additional sources of nutrients. Nigeria, and indeed, all of Africa, has a rich diversity of plant food sources which have been grossly underutilized. From time immemorial, fruits and vegetables have played significant roles in meeting the nutritional needs of animals and humans, thus improving global nutrition and wellness (Gul et al., 2016). Plant fruits constitute a rich source of the macro and micronutrients required for animal growth and development (Saxena et al., 2013). Thus, the exploration and evaluation of the nutritional potential of indigenous wild fruits and vegetables will not only reduce hunger but also improve global nutrition and wellness.

Blighia sapida is an herbaceous perennial plant native to tropical West and Central Africa. It is a member of the Sapidaceae family. In Nigeria and Jamaica, where B. sapida fruit forms a major delicacy, it is commonly called ackee. Ackee apple is consumed not only for nutrition but also for disease prevention (Adarkwa-Yiadom, 2018; Lawal et al., 2018). The potential of ackee apple to promote health and well-being has led to a recent increase in research on this grossly underused plant, especially in Nigeria and neighboring West African states where ackee is a staple food. In Jamaica, however, the ackee industry contributed a significant US$ 400 million in revenue in 2005 (Ekué et al., 2011). Thus, ackee has great economic potential in countries like Nigeria, especially with the recent push to open the non-oil sector. Ackee fruit is consumed both fresh and cooked. In addition, products such as soap made from ackee provide substantial revenue to peasant farmers, especially women (Ekué et al., 2010; Adeduntan et al., 2016).

Immature ackee fruit can be somewhat toxic. Symptoms such as vomiting, drowsiness and even hyperglycemia have been reported due to acute toxic effects associated with the consumption of unripe ackee aril fruit (Manchester, 1974). In addition, there has been reported cases of coma and death occurring within 12 h in severe cases of immature ackee aril poisoning (Brown et al., 1991). This is due to the presence of hypoglycin A, an unusual amino acid (Bowen-Forbes and Minott, 2011). However, as the ackee fruit matures, its hypoglycin A is converted to a less toxic hypoglycin B. In addition, proper harvesting and processing techniques, such as cooking of the mature fruit, have also been shown to reduce the hypoglycin level in the fruit by leaching hypoglycin A (Golden et al., 1984; Blake et al., 2006).

Antioxidants are substances that impede the oxidation of biomolecules either by extinguishing free radicals or chelating the metals (Arina and Harisun, 2019). Antioxidants protect against the deleterious effects of free radicals. They also neutralize the adverse effects of oxidative stress (Asadi et al., 2017). Polyphenols found in both edible and non-edible plants have been found to have antioxidant activity (Hoba et al., 2018). This is mainly due to their ability to scavenge and neutralize the various forms of free radicals. B. sapida aril has been demonstrated to have as much as 15% (w/w) oil content. Thus, B. sapida fruit could be properly classified as an oil-bearing fruit with a rich content of both saturated and unsaturated fatty acids (Hoba et al., 2018).

Drying as a preservation technique is most often employed in the dehydration of food, thus reducing its susceptibility to microbial spoilage. This specific method of preservation has been shown to affect the quality of food, including its physicochemical and sensorial properties as well as microbiological attributes. The use of drying methods, such as sun drying and oven drying, in the dehydration of B. sapida arils has been reported by some authors (Akintayo et al., 2002; Oyeleke et al., 2013), and the effect of different drying methods on other agricultural products has been widely reported by several others. However, there exists a dearth of information on the effects of different drying techniques on the nutritional quality of dried B. sapida arils. The present study was therefore designed to provide information on the comparative effects of sun- and oven-drying methods in the preservation of B. sapida aril on the composition of its flour as well as the nutritional and antioxidant properties of its oil extract.

2 Materials and methods

2.1 Chemical and reagents

All chemicals and reagents were of analytical grade and were products of Sigma-Adrich (UK) unless otherwise stated.

2.2 Plant material

Fresh B. sapida fruits were harvested in the month of August from an ackee apple plant at Ikare Akoko, Ondo State, Nigeria. The arils were immediately removed from the pods, seeds and raphe of the fruits; washed with copious water and transported in dark cellophane bags to the laboratory for further analysis. The leaves and fruits of the plant sample were taken to the Department of Plant Science, University of Ilorin (Nigeria) for authentication.

The ackee apple arils were divided into two equal halves. A portion was dried in the sun for five consecutive days (120 h) at 33 °C (Ayanwale et al., 2007) and labelled sun-dried apple aril (SDA). The second portion was dried in an oven (Gallenkamp OV-300, England) at 50 °C for 48 h.

2.3 Oil extraction

The sun-dried and oven-dried B. sapida arils were separately milled into fine powder using a kitchen blender and the powdered samples kept separately in an airtight dark container and kept at 4 °C until required for further analysis. A known sample (50 g) of each powder was extracted with n-hexane (1:5; flour:solvent) at 65–68 °C for 6 h using a Soxhlet apparatus (Karthikeyan et al., 2017). The extracted oil was dried using a rotary evaporator and then stored in a dark air-tight container. The percentage oil yield was calculated using the equation below: equation Where W₁ is the weight of the oil and W₂ the weight of the dried B. sapida flour sample.

2.4 Proximate composition

The proximate composition of the fresh and defatted oven-dried and sun-dried B. sapida aril flours was determined using AOAC methods (2005).

2.5 GC-MS analysis

Samples of both fresh and defatted B. sapida aril flour were each separately pulverized and mixed with GC-grade methanol (1:5 w/v). The mixture was placed in an orbital shaker and left at 25 ± 2 °C for 48 h. Thereafter, the mixture was filtered through a muslin cloth to obtain the crude extract which was concentrated using a rotary evaporator and stored at −4 °C until required for further analysis. The methanol extract of both defatted flour for both sun-dried and over-dried B. sapida aril were each separately subjected to GC-MS analysis in order to identify its constituent bioactive metabolites. The detection was at 70 eV ionization energy and 60 kPa with helium as the carrier gas. The initial temperature of the oven was set at 100 °C for 2 min and ramp rate of 4 °C per min to 225 °C and then 1 °C per min to 245 °C followed by 40 to 280 °C with a 30 min hold. An injection volume of 2 μL was injected at 280 °C with a split ratio of 1:50. Detected spectra were identified from the NIST database using the ChemStation software. Following the extraction process prior to GC-MS analysis, the extract was derivatized with N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) reagents. The resulting trimethylsilyl derivatives were separated and quantified using GC-MS (Stalikas, 2007).

2.6 Fatty acid analysis

The fatty acid profile of the B. sapida aril oil was determined using gas chromatography as described by Oluba et al. (2008).

2.6.1 Physicochemical properties

The refractive index and specific gravity of the oil samples were determined using a refractometer and a universal hydrometer respectively. The iodine value was evaluated according to the procedure described by Strong and Kosh (1974). Acid and peroxide values were estimated following the methods of Li et al. (2007) and Yildiz et al. (2003), respectively while the saponification number was evaluated based on the method of Dileesh et al. (2013). The free fatty acid content of the oil samples was determined using the titrimetric method of Kardash and Tur (2005) using phenolphthalein as indicator.

2.7 Total phenolic content determination

Total phenol content of the oil samples was determined as reported by Waterhouse (2002) and its content was calculated and recorded as gallic acid equivalent (GAE).

2.8 Antioxidant analysis

2.8.1 DPPH scavenging activity

The DPPH scavenging activity of the oil sample was carried out following the procedures of Bersuder et al. (1998) as described by Oluba et al. (2020). The DPPH scavenging capacity was estimated according to the equation: equation

Acontrol = Absorbance of the ethanolic DPPH solution;

Asample = Absorbance of oil sample or vitamin C in ethanolic DPPH solution.

The sample concentration required to scavenge 50% DPPH radical was evaluated from the vitamin C standard curve.

2.8.2 Nitric oxide scavenging activity

The oil samples were evaluated for their potential to scavenge nitric oxide according to the procedure detailed by Makhija et al. (2011). The procedure is based on the generation of nitric oxide radical (NO*) due to the decomposition of sodium nitroprusside in aqueous solution at pH 7.2. The NO* then reacts with oxygen to form nitrite and nitrate whose concentration is determined using Griess’s reagent (2008).

2.8.3 Lipid peroxidation

The scavenging activity of the oil samples against lipid peroxides (herein measured as thiobarbituric acid reactive species, TBARS) generated by ferric sulphate in egg yolk homogenate was assayed using the method described by Okoh et al. (2014). Briefly, 0.1 mL of varying concentration (0.05 to 0.5 mL) of the oil sample or vitamin C dissolved in DMSO as the case may be, was added to 0.5 mL of egg yolk homogenate (10%) and the resulting mixture made up to 1.0 mL. Lipid peroxidation was then induced by the addition of 0.5 mL ferric sulphate (0.07 M). The mixture was incubated for 30 min and 1.5 mL each of 10% acetic acid (pH 3.5) and 0.08% 2-thiobarbituric acid (in 1.1% sodium dodecyl sulphate and 20% trichloroacetic acid) was added. The reaction mixture was vortexed for 5 min, heated at 65 °C for one hour and then allowed to cool. To the cooled solution, 0.5 mL of n-butanol was added and the resulting mixture centrifuged at 3000 rpm for 10 min. The absorbance of the upper organic layer was read at 532 nm.

2.9 Statistical analysis

Results are reported as mean ± SD and statistically analyzed using One-Way Analysis of Variance (ANOVA) followed by Turkey’s multiple comparisons. Confidence value was set at 95%.

3 Results

3.1 Proximate composition

In terms of the effect of the drying method on crude protein level, the oven-dried flour had significantly (p > 0.05) lower protein content compared to the sun-dried flour. However, considering the impact of defatting on B. sapida aril, the defatted flour at both drying instances had significantly (p > 0.05) higher crude protein content compared to the flour. The oven-dried aril flour also contained significantly (p > 0.05) lower fat compared to the sun-dried aril flour. The amount of crude fibre obtained in the defatted flours (for both oven-dried and sun-dried) was significantly (p > 0.05) higher compared to the fresh oven-dried aril flour (Tab. 1).

Table 1

Proximate composition analysis of both fresh and defatted oven-dried and sun-dried B. sapida aril flours.

3.2 Phytochemicals composition

GC-MS analysis of the fresh B. sapida aril revealed the presence of pregn-4-ene-3,20-dione,17,21-dihydroxy-, bis(O-methyloxime) and 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one,8-(acetyloxy)-1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)- in the oven-dried flour while the sun-dried flour contained N-Methylpiperidin-2-yliden-3-morpholino-2-(4-chlorphenyl)-thioacrylamide, 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one,8 (acetyloxy)1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)-, Hexadecanoic acid, 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11-oxo-1H-2,8a-methanocyclopenta[a]cyclopropa[e]cyclodecen-6-yl ester, [1aR-(1aα,2α,5β,5aβ,6β,8aα,9α,10aα)]- and cyclopropanebutanoic acid, 2-[[2-[[2-[(2-pentylcyclopropyl)methyl]cyclopropyl]methyl]cyclopropyl]methyl]-, methyl ester. 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one,8-(acetyloxy)-1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)- was observed as a common component in both fresh oven- and sun-dried B. sapida aril flour (Fig. 1a). The defatted B. sapida aril flour showed the presence of ethyl iso-allocholate in the oven-dried sample while the sun-dried sample revealed the presence of tetradecanoic acid, 3,3a,4,6a,7,8,9,10,10a,10b-decahydro-3a,10a-dihydroxy-5-(hydroxymethyl)-2,10-dimethyl-3-oxobenz[e]azulen-8-yl ester, [3aR-(3aα,6aα,8α,10β,10aβ,10bβ)]- and pregn-4-ene-3, 20-dione, 17,21-dihydroxy-, bis (O-methyloxime) (Fig. 1b). These diverse chemicals belong to different chemical groups ranging from steroids, phytosterols, polyphenolics, terpenes, fatty esters, alkaloids etc. For instance, Ethyliso-allocholate is a triterpene; Pregn-4-ene-3,20-dione, 17,21-dihydroxy-, bis(O-methyloxime) is a steroid etc. The phytochemical components of the respective samples are indicated in Table 2.

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Fig. 1a

Pahytochemical contituents of fresh oven-dried (a and b) and sun-dried (w, x, y and z) B. sapida aril flour as determined using GC-MS analysis. Note: a: Pregn-4-ene-3,20-dione,17,21-dihydroxy-, bis(O-methyloxime); b: 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one, 8-(acetyloxy)-1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)-; w: N-Methylpiperidin-2-yliden-3-morpholino-2-(4-chlorphenyl)-thioacrylamide; x: 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one,8 (acetyloxy)1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)-; y: Hexadecanoic acid, 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11-oxo-1H-2,8a-methanocyclopenta[a]cyclopropa[e]cyclodecen-6-yl ester, [1aR-(1aα,2α,5β,5aβ,6β,8aα,9α,10aα)]-; z: Cyclopropanebutanoic acid, 2-[[2-[[2-[(2-pentylcyclopropyl)methyl]cyclopropyl]methyl]cyclopropyl]methyl]-, methyl ester.

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Fig. 1b

Pbhytochemical constitutents of defatted oven-dried (p) and sun-dried (q and r) B. sapida aril flour as determined using GC-MS analysis. Note: p: Ethyl iso-allocholate; q: Tetradecanoic acid, 3,3a,4,6a,7,8,9,10,10a,10b-decahydro-3a,10a-dihydroxy-5-(hydroxymethyl)-2,10-dimethyl-3-oxobenz[e]azulen-8-yl ester, [3aR-(3aα,6aα,8α,10β,10aβ,10bβ)]-; r: Pregn-4-ene-3,20-dione, 17,21-dihydroxy-, bis(O-methyloxime).

Table 2

GC-MS data analysis of the phytochemical components of both fresh and defatted oven-dried and sun-dried B. sapida aril.

3.3 Oil yield and fatty acid composition

The calculated yields of oil were 31.3 and 38.0% for the oven-dried and sun-dried B. sapida aril flours, respectively. Analysis of the fatty acid composition of the oven-dried B. sapida aril oil showed 64.60% saturated fatty acid, 33.95% monounsaturated fatty acid and 1.45% polyunsaturated fatty acid. The major saturated fatty acid component is palmitic acid (22.51 mg/L), myristic acid (16.74 mg/L) and lignoceric acid (1.98 mg/L) (Fig. 2a). Fatty acid profile of the sun-dried B. sapida oil showed 89.65% saturated fatty acid, 9.13% monounsaturated fatty acid, and 1.23% polyunsaturated fatty acid. The major saturated fatty acids are palmitic acid (52.22 mg/L), myristic acid (37.71 mg/L), lignoceric acid (3.07 mg/L), and arachidic acid (1.80 × 10⁻¹ mg/L). The monounsaturated fatty acids present are myristoleic acid (7.74 mg/L), gondoic acid (1.03 mg/L), palmitoleic acid (3.44 × 10⁻¹ mg/L), erucic acid (2.41 × 10⁻¹ mg/L) and oleic acid (1.28 × 10⁻¹ mg/L) while the following polyunsaturated acids were present docosahexaenoic acid (7.67 × 10⁻¹ mg/L), linolenic acid (1.79 × 10⁻¹ mg/L), 20:2 (1.74 × 10⁻¹ mg/L) and linoleic acid (1.55 × 10⁻¹ mg/L) (Fig. 2b).

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Fig. 2

Fatty acid composition of (a) oven-dried and (b) sun-dried B. sapida aril oil.

3.4 Physicochemical characteristics

The physicochemical characteristics of the oven-dried and sun-dried B. sapida aril oil were similar except for the free fatty acid and total phenolic contents that were significantly (p > 0.05) lower in the oven-dried oil sample compared to the sun-dried oil sample (Tab. 3). The two oil samples were yellow in colour with refractive index of 1.21 ± 0.02 and 1.23 ± 0.02 for oven-dried and sun-dried B. sapida oils respectively.

Table 3

Physicochemical characteristics of oven-dried and sun-dried B. sapida aril oil.

3.5 Antioxidant activity

Both the sun-dried and oven-dried aril oil displayed significant DPPH scavenging activity (Fig. 3a), lipid peroxide inhibitory activity (Fig. 3b) and nitric oxide scavenging activity (Fig. 3c). The oven-dried aril oil showed a lower significant (p < 0.05) DPPH scavenging and nitric oxide inhibitory activities compared to the sun-dried aril oil. The scavenging effects on both DPPH and nitric oxide radical were dose-dependent. The sun-dried aril oil and oven-dried aril oil displayed similar lipid peroxide scavenging activity especially at higher concentration (Fig. 3b). Overall, vitamin C demonstrated superior scavenging potential against DPPH, nitric oxide and lipid peroxide radicals compared to both oven-dried aril oil and sun-dried aril oil.

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Fig. 3

DPPH scavenging activity (a) lipid peroxide inhibitory activity (b) and nitric oxide scavenging activity (c) of sun-dried and oven-dried B. sapida aril oil in comparison to vitamin C. Results are means ± SD of three determinations. Note: SDAO: sun-dried aril oil; ODAO: oven-dried aril oil; vit C: vitamin C.

4 Discussion

The study on the composition of plants and plant products forms the basis for successful technological processing and applications of such plants (Saenz, 2000). The previous study on the proximate composition analyses of B. sapida arils from Toukountouna (Northwest Benin) showed that it contains 46% of crude fat, 47% of crude fibers and 3% of crude proteins (Dossou et al., 2004). The analysis of raw B. sapida arils from Mexico revealed 57.6% of moisture, 8.8% of crude protein, 18.8% of crude fat, 3.5% of crude fiber, 9.6% of total carbohydrates and 1.9% of ash (Morton, 1987). The crude protein values as reported for both oven-dried and sun-dried B. sapida flour in this study were higher than that reported by Dossou et al. (2004) and Morton (1987). In the same vein, results from the present study gave a crude fat level of 38.0% (for sun-dried flour) which was lower than the reported values by Dossou et al. (2004) and Morton (1987). This goes to show that the proximate composition of B. sapida flour could possibly vary from place to place. In addition, the present study demonstrated that the sun-dried B. sapida flour had considerably higher contents of protein and oil compared to oven- dried flour. Thus, in addition to the influence of geographical location in determining the percentage crude protein and fat in B. sapida flour, the choice of drying method also plays a significant role. Judging by results of this study, sun drying might be the preferred drying method of choice in obtaining higher protein and oil quantities from B. sapida flour. Defatting has been shown to improve the nutritional and functional attributes of oilseed flour (Akinyede and Amoo, 2009; Ndie et al., 2010). A report by Ndie et al. (2010) showed that defatted African walnut flour had higher protein content compared with the non-defatted flour. Results from this study showed that the crude protein contents of B. sapida aril flour were significantly increased after defatting. The crude protein content obtained for the defatted oven-dried and sun-dried B. sapida aril flours in this study compared favorably with that of many known legume flours, including cowpea (24.1%), egusi melon (23.4%), pigeon pea (19.9–24.0%), and chicken pea (23.7%) (Ojieh et al., 2007; Maninder et al., 2007; Anderson-Foster et al., 2012; Sreerama et al., 2012). The crude protein content of defatted B. sapida aril flour as reported in the present study is sufficient to meet the minimum dietary protein recommendation of the Food and Agriculture Organization (FAO/WHO, 2002).

Phytochemicals also referred to as plant secondary metabolites play significant roles in the free radical scavenging activities of plants and animals. The antioxidant properties of plants secondary metabolites are attributed to the activity of the phytochemicals they contain (Saxena et al., 2013; Lawal et al., 2018). The GC-MS fingerprints of both fresh and defatted B. sapida aril flour revealed the presence diverse phytochemicals including triterpenes, sesquiterpenes, steroids, quinones, alkaloids, polyphenols, glycosides and phytosterols in accordance with previous studies by Antwi et al. (2009), Onuekwusi et al. (2014), Balogun and Fetuga (1988). Pregn-4-ene-3,20-dione, 17,21-dihydroxy-, bis(O-methyloxime) has been reported to exhibit anti-inflammatory activity as well as been used in hormone replacement therapy (Hameed et al., 2018), Tetradecanoic acid, 3,3a,4,6a,7,8,9,10,10a,10b-decahydro-3a,10a-dihydroxy-5-(hydroxymethyl)-2,10 -dimethyl-3-oxobenz[e]azulen-8-yl ester, [3aR-(3aα,6aα,8α,10β,10aβ,10bβ)] has also been documented to show anti-inflammatory activity (Mohammed et al., 2016). Cyclopropanebutanoic acid, 2-[[2-[[2-[(2-pentylcyclopropyl) methyl]cyclopropyl]methyl]cyclopropyl]methyl]-, methyl ester identified in the methanolic leaf extract of Cinnamomum iners was demonstrated to show strong antioxidant activity against reactive oxygen species (Udayaprakash et al., 2015). According to a report by Malathi and Ramaiah (2017), Ethyl iso-allocholate isolated from rice was demonstrated to exhibit antimicrobial acitivity against E. coli through the inhibition of dihydropteroate synthase.

Phenolic compounds, due to their electron releasing and hydrogen donating potentials, are responsible for the antioxidant activity of edible fruits and vegetables. The result obtained from the phytochemical screening of both fresh and defatted B. sapida aril flour showed the presence of phenolic compounds. Polyphenols, carotenoids and nitrogen containing compounds are classical examples of substances with antioxidant activities (Asiamah 2018; Musa et al., 2019). The high level of unsaturation in phenolic compounds makes them the preferred oxidation substrates for free radicals thus protecting intracellular molecules against oxidation. The phenolics component of B. sapida aril could thus account for its ability to scavenge free radicals in DPPH, nitric oxide and lipid peroxides as demonstrated in this study. In addition, the oven-dried and sun-dried aril oils contained as much as 35.40 and 10.36% unsaturated fatty acids, respectively, which could serve as potential electron donors. Data from this study showed that the sun-dried B. sapida flour showed the presence of a more diverse range of phenolic compounds compared to the oven-dried flour. However, oil obtained from the oven-dried flour showed a higher degree of unsaturation in terms of its fatty acid content than oil from the sun-dried flour. These observations could naturally translate to the sun-dried B. sapida oil exhibiting higher antioxidant ability than oil from the oven-dried B. sapida flour as was observed in the present study. The fatty acid profiles obtained from both oven-dried and sun-dried B. sapida aril oil in the study identified palmitic acid as the major fatty acids. This is in agreement with the report of Odutuga et al. (1992) who had earlier shown that palmitic acid was the major fatty acid in B. sapida aril from Jamaica. However, other studies have shown that the major fatty acid in B. sapida aril is oleic acid (Tsado et al., 2018; Grande-Tovar et al., 2019). The differences in geographic location as well as processing methods could account for the different results.

In agreement with previous reports the oil obtained from both oven-dried and sun-dried B. sapida aril in this study is yellow in colour (Djenontin et al., 2009). The refractive index of 1.21 obtained for oven-dried B. sapida oil in this study is low compared to the value (1.46) obtained for B. sapida aril from Niger state, Nigeria. Refractive index of oil has been established to be a good predictor of oil susceptibility to rancidity. Oils with low refractive indices are less prone to oxidative rancidity (Godswill et al., 2018). Data on specific gravity obtained in the present study indicated that B. sapida aril oil is less dense compared to water. Oils generally have specific gravity of less than one. Reports from a study have demonstrated that B. sapida aril oil is less dense than oils from canola, cottonseed, and sesame (Dossou, 2014). The iodine values obtained for both sun-dried and oven-dried B. sapida aril in this study is similar to the reported value for palm oil but lower than that of groundnut oil, soybean oil and egusi melon (Oluba et al., 2008). The low iodine value reported for B. sapida aril oil in this study shows that it’s a non-drying oil. The iodine value of oils gives a measure of their degree of unsaturation as well as their susceptibility to oxidative rancidity (Oyeleke et al., 2013; Adepoju et al., 2013). The acid values of 3.31 and 3.57 mgKOHg⁻¹ obtained for oven-dried and sun-dried B. sapida aril oil, respectively in this study is low compared to the reported value for corn oil and peanut oil which are majorly utilized for pharmaceutical products. Thus, B. sapida aril oil could find possible application in the pharmaceutical industries. Its low acid value also showes that it is safe for consumption (Howele et al., 2010). The saponification values of oven-dried and sun-dried B. sapida aril oil obtained in this study compare relatively to that of most plant oils used in soap making (Oluba et al., 2008; Anderson-Foster et al., 2012). This justified its suitability in soap making in countries like Nigeria and Benin (Ekué et al., 2010). Overall, the physicochemical characteristics results obtained for both oven-dried and sun-dried B. sapida aril oil in this study is higher than the values reported by Aloko et al. (2017).

The higher total phenolic content of the oil sample obtained from sun-dried B. sapida flour could possibly account for the observed higher capacity of the oils to scavenge DPPH radical when compared to oil from the oven-dried B. sapida flour. Phenolics have established free radical scavenging activity hence capable of minimizing oxidative degradation of biomolecules (Kang et al., 2005; Amoateng et al., 2010). According to the recommendation of Scalbert and Williamson (2000) the consumption of 1000 mgGAE/day of antioxidants is desirable thus the daily intake of 100 g of B. sapida oil is sufficient to meet this nutritional requirement. The stem back extract of B. sapida was recently demonstrated to show DPPH scavenging as well as iron chelating activities (Ojo et al., 2018). According to Oloyede et al. (2013), B. sapida aril polyphenolics was demonstrated to scavenge and detoxify free radicals thus impeding lipid and protein oxidation. In addition, the ethyl acetate extracts of B. sapida pod and seed were demonstrated to exhibit strong DPPH antioxidant activities (Parkinson, 2007).

5 Conclusion

Data obtained from this study showed that the chemical composition of Blighia sapida aril is greatly influenced both by geographical location and preservation method. Our data showed that sun-dried B. sapida flour contained higher crude protein and crude fat than oven-dried flour. In addition, the sun-dried B. sapida aril flour contained more diverse phytochemicals compared to the oven-dried aril flour. More so, oil from the sun-dried aril had significantly higher total phenolics content and thus displayed better antioxidant activity compared to the oven-dried aril oil. However, in vitro antioxidant assays do not always translate to antioxidant properties, therefore, to further ascertain this claim, in vivo studies are necessary. Therefore, the method of choice for drying will be greatly influenced by the intended application of either the flour or the oil. Based on results obtained from this study, sun-drying is recommended for both nutritional purposes and health-promoting usage such as antioxidant, over oven-drying.

Abbreviations

BSAF: Blighia sapida aril flour

ODAO: Oven dried aril oil

SDAO: Sun-dried aril oil

GC-MS: Gas chromatography-mass spectrometry

DPPH: 2,2-diphenyl-1-picrylhydrazyl

NO: Nitric oxide

Conflicts of interest

The authors declare no conflicts of interest.

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Cite this article as: Oluba OM, Mbamara D-FO, Akpor OB, Adebiyi FD, Alabi OO, Shoyombo A, Osemwegie OO. 2021. Effects of drying methods on compositional characterization and functional characteristics of Blighia sapida aril oil. OCL 28: 17.

All Tables

Table 1

Proximate composition analysis of both fresh and defatted oven-dried and sun-dried B. sapida aril flours.

In the text

Table 2

GC-MS data analysis of the phytochemical components of both fresh and defatted oven-dried and sun-dried B. sapida aril.

In the text

Table 3

Physicochemical characteristics of oven-dried and sun-dried B. sapida aril oil.

In the text

All Figures

thumbnail
Fig. 1a

Pahytochemical contituents of fresh oven-dried (a and b) and sun-dried (w, x, y and z) B. sapida aril flour as determined using GC-MS analysis. Note: a: Pregn-4-ene-3,20-dione,17,21-dihydroxy-, bis(O-methyloxime); b: 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one, 8-(acetyloxy)-1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)-; w: N-Methylpiperidin-2-yliden-3-morpholino-2-(4-chlorphenyl)-thioacrylamide; x: 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one,8 (acetyloxy)1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)-; y: Hexadecanoic acid, 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11-oxo-1H-2,8a-methanocyclopenta[a]cyclopropa[e]cyclodecen-6-yl ester, [1aR-(1aα,2α,5β,5aβ,6β,8aα,9α,10aα)]-; z: Cyclopropanebutanoic acid, 2-[[2-[[2-[(2-pentylcyclopropyl)methyl]cyclopropyl]methyl]cyclopropyl]methyl]-, methyl ester.

In the text

thumbnail
Fig. 1b

Pbhytochemical constitutents of defatted oven-dried (p) and sun-dried (q and r) B. sapida aril flour as determined using GC-MS analysis. Note: p: Ethyl iso-allocholate; q: Tetradecanoic acid, 3,3a,4,6a,7,8,9,10,10a,10b-decahydro-3a,10a-dihydroxy-5-(hydroxymethyl)-2,10-dimethyl-3-oxobenz[e]azulen-8-yl ester, [3aR-(3aα,6aα,8α,10β,10aβ,10bβ)]-; r: Pregn-4-ene-3,20-dione, 17,21-dihydroxy-, bis(O-methyloxime).

In the text

thumbnail
Fig. 2

Fatty acid composition of (a) oven-dried and (b) sun-dried B. sapida aril oil.

In the text

thumbnail
Fig. 3

DPPH scavenging activity (a) lipid peroxide inhibitory activity (b) and nitric oxide scavenging activity (c) of sun-dried and oven-dried B. sapida aril oil in comparison to vitamin C. Results are means ± SD of three determinations. Note: SDAO: sun-dried aril oil; ODAO: oven-dried aril oil; vit C: vitamin C.

In the text

Table 1

Proximate composition analysis of both fresh and defatted oven-dried and sun-dried B. sapida aril flours.

Composition (%)
Oven-dried aril flour
Sun-dried aril flour

Fresh sample
Defatted sample
Fresh sample
Defatted sample

Crude protein
15.03 ± 1.13a
19.50 ± 2.21b
21.88 ± 1.58b
26.40 ± 3.80c

Crude fat
31.30 ± 3.53a
–
38.00 ± 5.22b
–

Crude fibre
5.53 ± 0.55a
8.60 ± 0.55b
5.22 ± 0.95a
8.33 ± 1.11b

Ash
8.12 ± 0.88b
8.33 ± 1.22b
6.03 ± 1.10a
6.10 ± 0.53a

Total carbohydrate
11.12 ± 2.33a
11.73 ± 2.27a
12.12 ± 1.35a
12.33 ± 1.15a

Results are means ± SD of three determinations. Values in the same row carrying different alphabets are significant (p > 0.05).

Fig. 1a

Pahytochemical contituents of fresh oven-dried (a and b) and sun-dried (w, x, y and z) B. sapida aril flour as determined using GC-MS analysis. Note: a: Pregn-4-ene-3,20-dione,17,21-dihydroxy-, bis(O-methyloxime); b: 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one, 8-(acetyloxy)-1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)-; w: N-Methylpiperidin-2-yliden-3-morpholino-2-(4-chlorphenyl)-thioacrylamide; x: 4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one,8 (acetyloxy)1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a-dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)-; y: Hexadecanoic acid, 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11-oxo-1H-2,8a-methanocyclopenta[a]cyclopropa[e]cyclodecen-6-yl ester, [1aR-(1aα,2α,5β,5aβ,6β,8aα,9α,10aα)]-; z: Cyclopropanebutanoic acid, 2-[[2-[[2-[(2-pentylcyclopropyl)methyl]cyclopropyl]methyl]cyclopropyl]methyl]-, methyl ester.

Fig. 1b

Pbhytochemical constitutents of defatted oven-dried (p) and sun-dried (q and r) B. sapida aril flour as determined using GC-MS analysis. Note: p: Ethyl iso-allocholate; q: Tetradecanoic acid, 3,3a,4,6a,7,8,9,10,10a,10b-decahydro-3a,10a-dihydroxy-5-(hydroxymethyl)-2,10-dimethyl-3-oxobenz[e]azulen-8-yl ester, [3aR-(3aα,6aα,8α,10β,10aβ,10bβ)]-; r: Pregn-4-ene-3,20-dione, 17,21-dihydroxy-, bis(O-methyloxime).

Table 2

GC-MS data analysis of the phytochemical components of both fresh and defatted oven-dried and sun-dried B. sapida aril.

Sample
Molecular formula
Molecular weight (g)
Exact mass (g)
Probability
(%)
CAS number
NIST number
Name

Fresh oven-dried flour
C₂₃H₃₆N₂O₄
404
404.267508
30.9
55557-09-0
17078
Pregn-4-ene-3,20-dione, 17,21-dihydroxy-, bis(O-methyloxime)

C₂₂H₃₀O₈
422
422.194067
19.6
77646-23-2
67592
4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one, 8-(acetyloxy)-1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a −dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)

Fresh sun-dried flour
C₂₂H₃₀O₈
422
422.194067
19.6
77646-23-2
67592
4H-Cyclopropa[5’,6’]benz[1’,2’:7,8]azuleno[5,6-b]oxiren-4-one, 8-(acetyloxy)-1,1a,1b,1c,2a,3,3a,6a,6b,7,8,8a −dodecahydro-3a,6b,8a-trihydroxy-2a-(hydroxymethyl)-1,1,5,7-tetramethyl-, (1aα,1bβ,1cβ,2aβ,3aβ,6aα,6bα,7α,8β, 8aα)

C₃₆H₅₈O₆
586
586.42334
22.5
52557-26-3
67658
Hexadecanoic acid, 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11 −oxo-1H-2,8a-methanocyclopenta[a]cyclopropa[e]cyclodecen-6-yl ester, [1aR-(1aα,2α,5β,5aβ,6β,8aα,9α,10aα)]

C₂₅H₄₂O₂
374
374.318481
57.9
56051-53-7
28143
Cyclopropanebutanoic acid, 2-[[2-[[2-[(2-pentylcyclopropyl)methyl]cyclopropyl]methyl]cyclopropyl]methyl]-, methyl ester

C₁₉H₂₄ClN₃OS
377
377.13286
26.2
155243-50-8
286965
N-Methylpiperidin-2-yliden-3-morpholino-2-(4-chlorphenyl)-thioacrylamide

Defatted oven-dried flour
C₂₆H₄₄O₅
436
436.318874
31.4

43053
Ethyl iso-allocholate

Defatted sun-dried flour
C₃₁H₅₀O₆
518
518.36074
37.2
77058-95-8
67590
Tetradecanoic acid, 3,3a,4,6a,7,8,9,10,10a,10b-decahydro-3a,10a-dihydroxy-5-(hydroxymethyl)-2,10 −dimethyl-3-oxobenz[e]azulen-8-yl ester, [3aR-(3aα,6aα,8α,10β,10aβ,10bβ)]

C₂₃H₃₆N₂O₄
404
404.267508
10.3
55557-09-0
17078
Pregn-4-ene-3,20-dione, 17,21-dihydroxy-, bis(O-methyloxime)

Fig. 2

Fatty acid composition of (a) oven-dried and (b) sun-dried B. sapida aril oil.

Table 3

Physicochemical characteristics of oven-dried and sun-dried B. sapida aril oil.

Physicochemical parameter | ODAO | SDAO
Colour | Yellow | Yellow
Refractive index | 1.21 ± 0.02a | 1.23 ± 0.02a
Specific gravity (gL⁻¹) | 0.91 ± 0.02a | 0.90 ± 0.01a
Peroxide value (mg reactive O₂g⁻¹) | 9.55 ± 0.35a | 9.83 ± 0.30a
Acid value (mgKOHg⁻¹) | 3.31 ± 0.15a | 3.57 ± 0.19a
Saponification value (mgKOHg⁻¹) | 191.50 ± 7.23a | 196.35 ± 5.32a
Iodine value (mgI₂g⁻¹) | 90.33 ± 3.33a | 92.39 ± 2.80a
Free fatty acid (%) | 0.21 ± 0.01a | 0.35 ± 0.00b
Total phenolic content (mgGAEg⁻¹) | 2.57 ± 0.35a | 3.86 ± 0.30b

Results are means ± SD of three determinations. Values in the same row carrying different alphabets are significant (p > 0.05). Note: SDAO: sun-dried aril oil; ODAO: oven-dried aril oil; vit C: vitamin C; GAE: gallic acid equivalent.

Fig. 3

DPPH scavenging activity (a) lipid peroxide inhibitory activity (b) and nitric oxide scavenging activity (c) of sun-dried and oven-dried B. sapida aril oil in comparison to vitamin C. Results are means ± SD of three determinations. Note: SDAO: sun-dried aril oil; ODAO: oven-dried aril oil; vit C: vitamin C.